This NSF-funded project uses functional genomics to investigate the process of legume root hair infection by nitrogen-fixing rhizobia. This infection initiates the symbiosis between this bacterium and its host that will result in the de novo formation of a novel organ, the nodule. It is within the nodule that the bacterium fixes nitrogen providing its host plant an advantage in environments where this element is limiting. The establishment of the symbiosis involves a complex interplay between host and symbiont, which is orchestrated by the exchange of diffusible signal molecules.

 

 
 

Wan, J., M. Torres, A. Ganapathy, J. Thelen, B. B. DaGue, B. Mooney, D. Xu, and G. Stacey. (2005) "Proteomic analysis of soybean root hairs after infection by Bradyrhizobium japonicum", Mol. Plant-Microbe Int. 18: p. 458. DOI: 10.1094/MPMI-18-0458.

Stacey G, Libault M, Brechenmacher L, Wan J, May GD (2006) "Genetics and functional genomics of legume nodulation.” Curr. Opin. Plant Biol. 9: p. 110. doi:10.1016/j.pbi.2006.01.005 

Song, Z., Chen, L, Ganapathy, A., Wan, X-F, Brechenmacher, L., Tao, N., Emerich, D., Stacey, G, Xu, Dong (2007) "Development and assessment of scoring functions for protein identification using peptide mass fingerprinting data.", Electrophoresis 28: p. 864. DOI: 10.1002/elps.200600305

Stacey, G, McAlvin, C.B., Kim, S-Y, Olivares, J., Soto, M.J. (2006) "Effects of endogenous salicylic acid on nodulation in the model legumes Lotus japonicus and Medicago truncatula.", Plant Physiol. 141: p. 1473.  DOI: 10.1104/pp.106.08098

Subramanian S, Stacey G, Yu Oliver (2006) "Endogenous isoflavones are essential for the establishment of symbiosis between soybean and Bradyrhizobium japonicum", Plant Journal 48: p. 261. DOI: 10.1111/j.1365-313X.2006.02874.x

Subramanian S, Stacey G, Yu O (2007) "Distinct, critical roles of flavonoids during determinate and indeterminate legume nodulation." Trends in Plant Science 12: p. 282 . DOI: 10.1016/j.tplants.2007.06.006
NSF Functional Genomics of Root Hair Infection
university of missouri • columbia
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